Detection of Amino Acid Mutations in Dihydrofolate Reductase & Dihydro Pteroate Synthase Gene in Plasmodium Vivax Isolates

Authors

  • Rizwana Yasmin
  • Musarrat Jehan
  • Mehwish Kalam
  • Syeda Mariam Siddiqa
  • Mahadev Harani

Keywords:

Plasmodium vivax, pvdhfr gene, pvdhps gene, Antifolate drug resistance markers.

Abstract

Objective: To assess the frequency and patterns of mutations and distribution of alleles in Plasmodium. Vivax Dihydrofolate Reductase (dhfr) & Dihydro Pteroate Synthase (dhps) genes in Karachi.
Methodology: The descriptive cross-sectional study was carried out at the Department of Biotechnology, University of Karachi from June 2014 to February 2016. The sampling technique used was Non-probability convenient sampling method. A total of n=200 malaria cases irrespective of age and gender from Jinnah Medical College Hospital Korangi Karachi were selected. Around 200 µL of blood was collected in micro centrifuge tubes. Among them, 36 cases of P. vivax were detected by microscopy. DNA was extracted from these 36 samples followed by polymerase chain reaction (PCR) and sequencing of amplified genes of Plasmodium vivax Dihydrofolate reductase (pvdhfr) & Plasmodium vivax Dihydro pteroate synthase (pvdhp).
Results: The analysis of 36 amplified P. vivax isolates revealed presence of various alleles of pvdhfr and pvdhps genes producing both wild type (W) and mutant amino acids having single, double & multiple mutations at multiple positions. In pvdhfr gene, common mutations were observed in ‘S’ amino acids at codons 58 &117 and ‘D’ at codons 105 &157. Regarding pvdhps gene, common mutations were observed in ‘V’ amino acids at codon 398 and ‘K’ at codon 590.
Conclusion: The frequency and variety of mutations we detected in pvdhfr and pvdhps genes signify the drug resistance in plasmodium vivax so there is need of some measures to control drug resistance by better formulation using combination of drugs.

Downloads

Published

2020-07-21

How to Cite

1.
Yasmin R, Jehan M, Kalam M, Siddiqa SM, Harani M. Detection of Amino Acid Mutations in Dihydrofolate Reductase & Dihydro Pteroate Synthase Gene in Plasmodium Vivax Isolates. J Liaq Uni Med Health Sci [Internet]. 2020 Jul. 21 [cited 2024 Apr. 29];19(02):102-8. Available from: http://ojs.lumhs.edu.pk/index.php/jlumhs/article/view/345

Issue

Vol. 19 No. 02 (2020): Journal of Liaquat University of Medical & Health Sciences

Section

Original Articles

License

Submission of a manuscript to the journal implies that all authors have read and agreed to the content of the undertaking form or the Terms and Conditions.

When an article is accepted for publication, the author(s) retain the copyright and are required to grant the publisher the right of first publication and other non-exclusive publishing rights to JLUMHS.

Articles published in the Journal of Liaquat University of Medical & health sciences are open access articles under a Creative Commons Attribution-Noncommercial - Share Alike 4.0 License. This license permits use, distribution and reproduction in any medium; provided the original work is properly cited and initial publication in this journal. This is in accordance with the BOAI definition of open access. In addition to that users are allowed to remix, tweak and build upon the work non-commercially as long as appropriate credit is given and the new creations are licensed under the identical terms. Or, in certain cases it can be stated that all articles and content there in are published under creative commons license unless stated otherwise.

Most read articles by the same author(s)