Expression Profile of Apoptotic Genes Bak1 and Bcl2 in Pakistani Presbycusis Patients from Faisalabad and Chichawatni Populations
Keywords:
Presbycusis, age related hearing impairment, Apoptosis, BAK1, BCL2Abstract
Objective: To analyze the expression profiles of two apoptotic genes, BAK1 and BCL2, in Pakistani ARHI patients' blood samples compared to healthy subjects.
Methodology: This expression profiles study was conducted at the Sight Centre of Bahawalpur City of Southern Punjab from August 2022 to July 2023. All of the subjects were from Faisalabad or Chichawatni, Pakistan cities. The participants exposed to head injury or trauma, noise exposure, ototoxic medication, chemical exposure, diabetes, liver cirrhosis, neurological disease, psychiatric disease, cognitive dysfunction, middle ear disease, and brain tumour factors were excluded from the study. Individuals with cold or flu were also excluded from the study. RNA was extracted from both the peripheral blood samples of healthy and affected individuals. The quantity of RNA was then determined, and cDNA was synthesized from it. This cDNA was then utilized in qPCR to assess the relative expression of the BAK1, BCL2, and ACTB genes using gene-specific primers.
Results: Our results suggested that ARHI patients had higher levels of BAK1 expression and BAK1/BCL2 ratio than healthy subjects.
Conclusion: Thus, apoptosis mediated by BAK1 may be a key mechanism controlling the development of ARHI. Furthermore, changes in BAK1 gene expression of blood samples might be utilized as a rapid test for early diagnosis of ARHI.
References
Song W, Cao H, Zhang D, Xu H, Zhang Q, Wang Z et al. Association between NR3C1 gene polymorphisms and age-related hearing impairment in Qingdao Chinese elderly. BMC Med Genomics. 2021; 14(1): 193. doi: 10.1186/s12920-021-01044-4.
Duan H, Song W, Wang W, Cao H, Wang B, Liu Y et al. A Genome-Wide Association Study of Age-Related Hearing Impairment in Middle- and Old-Aged Chinese Twins. BioMed Res Int. 2021; 2021: 3629624. doi: 10.1155/2021/3629624.
Lau K, Dimitriadis PA, Mitchell C, Martyn-St-James M, Hind D, Ray J. Age-related hearing loss and mild cognitive impairment: a meta-analysis and systematic review of population-based studies. J Laryngol Otol. 2022; 136(2): 103-18. doi: 10.1017/S0022215121004114. Epub 2021 Dec 13.
Curhan SG, Wang M, Eavey RD, Stampfer MJ, Curhan GC. Adherence to Healthful Dietary Patterns Is Associated with Lower Risk of Hearing Loss in Women. J Nutr. 2018; 148(6): 944-51. doi: 10.1093/jn/nxy058.
Wang J, Puel JL. Presbycusis: An Update on Cochlear Mechanisms and Therapies. J Clin Med. 2020; 9(1): 218. doi: 10.3390/jcm9010218.
Huang Q, Tang J. Age-related hearing loss or presbycusis. Eur Arch Otorhinolaryngol. 2010; 267(8): 1179-91. doi: 10.1007/s00405-010-1270-7. Epub 2010 May 13.
Muderris T, Yar Sa?lam AS, Unsal D, Mülazimo?lu S, Sevil E, Kayhan H. Efficiency of resveratrol in the prevention and treatment of age-related hearing loss. Exp Ther Med. 2022; 23(1): 40. doi: 10.3892/etm.2021.10962. Epub 2021 Nov 12.
Ahmed S, Al-Râwanduzi A. Cellular and molecular mechanisms involved in age- related hearing loss with focusing on oxidative stress. Central Asian J Med Pharmaceut Sci Innov. 2022; 2: 66-75. doi: 10.22034/CAJMPSI.2022.02.04
Falah M, Najafi M, Houshmand M, Farhadi M. Expression levels of the BAK1 and BCL2 genes highlight the role of apoptosis in age-related hearing impairment. Clin Interv Aging. 2016; 11: 1003-8. doi: 10.2147/CIA.S109110.
Liu J, Chen H, Lin X, Yi J, Ye W, Wei F et al. Age-related Activation of Cyclic GMP-AMP synthase-Stimulator of Interferon Genes Signaling in the Auditory System is Associated with Presbycusis in C57BL/6J Male Mice. Neuroscience. 2022; 481: 73-84. doi: 10.1016/j.neuroscience.2021.11.031. Epub 2021 Nov 27.
Liu H, Li F, Li X, Wu Q, Dai C. Rapamycin ameliorates age-related hearing loss in C57BL/6J mice by enhancing autophagy in the SGNs. Neurosci Lett. 2022; 772: 136493. doi: 10.1016/j.neulet.2022.136493. Epub 2022 Jan 31.
Fetoni AR, Pisani A, Rolesi R, Paciello F, Viziano A, Moleti A et al. Early Noise-Induced Hearing Loss Accelerates Presbycusis Altering Aging Processes in the Cochlea. Front Aging Neurosci. 2022; 14: 803973. doi: 10.3389/fnagi.2022.803973.
Kishimoto-Urata M, Urata S, Fujimoto C, Yamasoba T. Role of Oxidative Stress and Antioxidants in Acquired Inner Ear Disorders. Antioxidants(Basel). 2022; 11(8): 1469. doi: 10.3390/antiox11081469.
Lin FR, Thorpe R, Gordon-Salant S, Ferrucci L. Hearing loss prevalence and risk factors among older adults in the United States. J Gerontol A Biol Sci Med Sci. 2011; 66A(5): 582-90. doi: 10.1093/gerona/glr002.
Ohlemiller KK, Wright JS, Dugan LL. Early elevation of cochlear reactive oxygen species following noise exposure. Audiol Neurootol. 1999; 4(5): 229-36. doi: 10.1159/000013846.
Hwang JH, Chen JC, Hsu CJ, Yang WS, Liu TC. Plasma reactive oxygen species levels are correlated with severity of age-related hearing impairment in humans. Neurobiol Aging. 2012; 33(9): 1920-6. doi: 10.1016/j.neurobiolaging.2011.10.012. Epub 2011 Dec 1.
Kamogashira T, Fujimoto C, Yamasoba T. Reactive oxygen species, apoptosis, and mitochondrial dysfunction in hearing loss. BioMed Res Int. 2015; 2015: 617207. doi: 10.1155/2015/617207. Epub 2015 Mar 22.
Westphal D, Dewson G, Czabotar PE, Kluck RM. Molecular biology of Bax and Bak activation and action. Bioch Biophys. 2011; 1813(4): 521-31. doi: 10.1016/j.bbamcr.2010.12.019. Epub 2010 Dec 30.
Cosentino K, García-Sáez AJ. Bax and Bak Pores: Are We Closing the Circle? Trends Cell Biol. 2017; 27(4): 266-75. doi: 10.1016/j.tcb.2016.11.004. Epub 2016 Dec 5.
Singh G, Guibao CD, Seetharaman J, Aggarwal A, Grace CR, McNamara DE et al. Structural basis of BAK activation in mitochondrial apoptosis initiation. Nature Commun. 2022; 13(1): 250. doi: 10.1038/s41467-021-27851-y.
Brooks C, Wei Q, Feng L, Dong G, Tao Y, Mei L et al. Bak regulates mitochondrial morphology and pathology during apoptosis by interacting with mitofusins. Proc Natl Acad Sci U S A. 2007; 104(28): 11649-54. doi: 10.1073/pnas.0703976104. Epub 2007 Jul 2.
Falah M, Najafi M, Houshmand M, Farhadi M. Expression levels of the BAK1 and BCL2 genes highlight the role of apoptosis in age-related hearing impairment. Clin Interv Aging. 2016; 11: 1003-8. doi: 10.2147/CIA.S109110.
Shin M, Pandya M, Espinosa K, Telang R, Boix J, Thorne PR et al. Istradefylline Mitigates Age-Related Hearing Loss in C57BL/6J Mice. Int J Mol Sci. 2021; 22(15): 8000. doi: 10.3390/ijms22158000.
Someya S, Xu J, Kondo K, Ding D, Salvi RJ, Yamasoba T et al. Age-related hearing loss in C57BL/6J mice is mediated by Bak-dependent mitochondrial apoptosis. Proc Natl Acad Sci U S A. 2009; 106(46): 19432-7. doi: 10.1073/pnas.0908786106. Epub 2009 Nov 9.
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