Genetic Association of DPP4 C/T and GLP1R C/A with Cytokines and Hormones in Undiagnosed Diabetic Subjects
Abstract
OBJECTIVE: Genetic relationship with biochemical, hormones and markers of inflammation.
METHODOLOGY: This collaborative study was cross-sectional and carried out by the Department of
Biochemistry, Karachi University, Baqai Diabetology and Endocrinology Institute (BIDE), Karachi Pakistan and Kansai Electric Power Institute of Medical Research (KEPIIMR), Osaka-Japan. The duration of the research was from March 2019 to May 2020. Both genders aged 30-70 (years) visiting the outpatient department of BIDE and fulfilling the inclusion criteria were recruited. An oral glucose tolerance test as diagnostic criteria was used. Subjects were categorized into healthy (glucose levels of fasting glucose (FPG) less than 100 (mg/dL), a two-hour glucose post level (2-hr PGL) below 140mg/dL). Newly diagnosed diabetics (NDD) (FPG ?126mg/dL or 2-hr PGL ?200mg/dL or both without any antidiabetic medicines use). Subjects with any complications were excluded. SPSS (Social Science Package for Statistical) Version 20 was used to analyze data.
RESULTS: Of 34 subjects, 17 were NDD and 17 were healthy. The mean age was 49.65±1.95, and the
mean BMI was 28.12±0.93. DPP-4 C/T exhibited significant findings (p<0.05) compared with GLP-1R C/A among healthy and NDD subjects. Polymorphism of GLP1R C/A is associated significantly with fasting triacylglycerides and GLP1. In Comparison, polymorphism of DPP4 C/T was associated significantly in fasting with GLP1, GIP, HDL and IL-1? when compared to NDD with healthy participants.
CONCLUSION: We found significantly more association of DPP4 C/T polymorphism rs2970932 with
biochemical parameters than GLP1R C/A polymorphisms rs1042044 in NDD individuals.
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