Serological Investigation and Molecular Characterization of Reticuloendotheliosis Virus from Breeder Flocks in Pakistan by Polymerase Chain Reaction
Keywords:
Reticuloendotheliosis virus, Polymerase chain reaction, PakistanAbstract
OBJECTIVE: The goal of this study was to provide the authentic information about the incidence of reticuloendotheliosis virus in Pakistan because several epidemiological studies suggested a link between avian oncogenic viruses and different types of cancers in people working in poultry industry.
METHODOLOGY: Cross sectional study to determine the REV infection status. Presence of antibodies in serum were observed using ELISA and confirmation of REV genome was made by polymerase chain reaction using long terminal repeat specific primers to amplify a 291bp fragment of REV viral genome from tumor samples of liver collected from breeder flocks with average age of 30 weeks. In all 480 serum and liver samples were taken from 24 poultry breeder across Punjab and Khyber Pakhtunkhwa.
RESULTS: Out of 480 serum samples 42 serum samples which were found positive for REV antibodies by ELISA. When subjected to PCR revealed n=37 to bear REV genome.
CONCLUSION: It is concluded that reticuloendotheliosis virus is present in breeder flocks across a wide range of area across Pakistan which can be hazardous to public health.
Downloads
Published
How to Cite
Issue
Section
License
Submission of a manuscript to the journal implies that all authors have read and agreed to the content of the undertaking form or the Terms and Conditions.
When an article is accepted for publication, the author(s) retain the copyright and are required to grant the publisher the right of first publication and other non-exclusive publishing rights to JLUMHS.
Articles published in the Journal of Liaquat University of Medical & health sciences are open access articles under a Creative Commons Attribution-Noncommercial - Share Alike 4.0 License. This license permits use, distribution and reproduction in any medium; provided the original work is properly cited and initial publication in this journal. This is in accordance with the BOAI definition of open access. In addition to that users are allowed to remix, tweak and build upon the work non-commercially as long as appropriate credit is given and the new creations are licensed under the identical terms. Or, in certain cases it can be stated that all articles and content there in are published under creative commons license unless stated otherwise.