Evaluation of the Role of Oxidative Stress and Inflammation in the Causation of Type 2 Diabetes Mellitus in Human Subjects

Authors

  • Tayyab uddin Khand University of Sindh
  • Naseem Aslam Channa
  • Fatehuddin Khand

Abstract

Objective: To inspect the impact of oxidative stress and inflammation on the   causation of type 2 diabetes mellitus in human subjects.

Methodology: ACase - control study on 200 randomly enrolled subjects (age range 20-40 years), including 100 T2 DM patients (56 males and 44 females) and 100 healthy controls (60 males and 40 females) matched for socioeconomic status were randomly enrolled from and were inhabitants of Hyderabad and Jamshoro districts of Sindh province, this research was conducted at postgraduate research laboratory of Institute of Biochemistry, University of Sindh Jamshoro from January-December 2019. Serum samples of the subjects were analyzed by kit methods for the amounts of creatinine, uric acid, total antioxidants, iron, C-reactive protein, superoxide dismutase, catalase, glutathione peroxidase, xanthine oxidase, lactate dehydrogenase, malondialdehyde and nuclear factor kappa- B. Urine samples collected on spot were investigated for pH and protein content by using urine dip strip automatic reader “Urisys 1100* Roche”.

Results: Physical measurements disclosed that obesity was more common in diabetic patients (26%) than in controls (10%). Serum analysis results showed that T2 DM patients as against the controls had significantly raised superoxide dismutase (p<0.01) and C-reactive protein (p<0.005); and decreased uric acid (p<0.006), iron (p<0.01) and xanthine oxidase (p<0.04) levels. Spot urine analysis results showed that controls compared to T2 DM patients excreted significantly (p<0.003) more acidic urine.

Conclusion: From the results of this study, we concluded that oxidative stress together with low grade inflammation plays an important role in the causation of T2 DM.

Keywords: Diabetes Mellitus, Antioxidants, Inflammation, Oxidative Stress

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Published

2021-07-02

How to Cite

1.
Khand T uddin, Channa NA, Khand F. Evaluation of the Role of Oxidative Stress and Inflammation in the Causation of Type 2 Diabetes Mellitus in Human Subjects. J Liaq Uni Med Health Sci [Internet]. 2021 Jul. 2 [cited 2024 May 4];20(02):113-5. Available from: http://ojs.lumhs.edu.pk/index.php/jlumhs/article/view/367

Issue

Vol. 20 No. 02 (2021): Journal of Liaquat University of Medical & Health Sciences

Section

Original Articles

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